PCR Supplies

A variety of products designed for use in various PCR procedures. Includes dNTPs, enzymes, assays, arrays, master mixes, reagents, and kits. Products can be used for standard, real-time, direct, high fidelity, hot start, and long range PCR protocols.

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106 – 120 of 4,526 results

106

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Applied Biosystems™ AmpliTaq™ DNA Polymerase, LD (Low DNA)

Applied Biosystems AmpliTaq DNA Polymerase, LD (Low DNA), is the same enzyme as AmpliTaq DNA Polymerase, but is further purified through a proprietary separation process to ensure that residual bacterial DNA sequences are substantially reduced.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	1X
Purity or Quality Grade	LD (Low DNA)
Quantity	250 units
Overhang	3'-A
Concentration	5 U/μL
For Use With (Application)	Standard PCR
Exonuclease Activity	5' - 3'
Polymerase	AmpliTaq DNA Polymerase
No. of Reactions	200 Reactions
Format	Stand-alone enzyme
Size (Final Product)	5 kb or less
Reaction Format	Separate Components
Product Type	DNA Polymerase
Reaction Speed	Standard
Hot Start	No
Starting Material	DNA

107

Invitrogen™ Cells-to-cDNA™ II Cell Lysis Buffer (10 ml Bottle)

The same lysis buffer found in the Invitrogen Cells-to-cDNA™ II Kit. Supplied in five 10 mL bottles.

Pricing & Availability

108

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Applied Biosystems™ MycoSEQ™ Plus Mycoplasma Detection Kit

The MycoSEQ™ Plus Mycoplasma Detection Kit is a TaqMan™-based quantitative PCR (qPCR) kit for the detection of mycoplasma in complex bioproduction samples.

Pricing & Availability

109

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Applied Biosystems™ ViralSEQ™ Lentivirus Physical Titer Kits

A one-step RT-qPCR assay for the quantitation of genome-containing lentiviral particles in supernatants from cell-based bioproduction systems. Lentiviral quantitation is a critical quality attribute (CQA) in viral vector manufacturing for cell and gene-modified cell therapy development.

Pricing & Availability
Specifications

Target	Lentivirus genomic target
For Use With (Equipment)	Real-time PCR instruments (QuantStudio5 or 7500 Fast)
Quantity	100 reactions
For Use With (Application)	Virus Detection
Detection Method	Primer-probe

110

Thermo Scientific™ Maxima H Minus Double-Stranded cDNA Synthesis Kit

The Thermo Scientific Maxima H Minus Double-Stranded cDNA Synthesis Kit is a complete system for efficient synthesis of double-stranded cDNA from total RNA or mRNA.

Pricing & Availability
Specifications

Reverse Transcriptase	Maxima H Minus
Reaction Format	Separate Components
Final Product Type	Double Stranded cDNA
Product Type	cDNA Synthesis Kit
Sample Type	RNA
For Use With (Application)	Cloning
Product Line	Maxima

111

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Applied Biosystems™ AmpliTaq™ DNA Polymerase with Buffer I or Buffer II

AmpliTaq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Fidelity (vs. Taq)	1X
Overhang	3'-A
Concentration	5 U/μL
For Use With (Application)	Standard PCR
Exonuclease Activity	5' - 3'
Polymerase	AmpliTaq DNA Polymerase
Format	Stand-alone enzyme
Size (Final Product)	5 kb or less
Reaction Format	Separate Components
Product Type	DNA Polymerase
Reaction Speed	Standard
Hot Start	No
Starting Material	DNA

112

Thermo Scientific™ SureTect™ Cronobacter species PCR assay

Simply and accurately detect Cronobacter species in low or large sample sizes of powdered infant formula, and related ingredients, and production environment samples in less than 20 hours with the Thermo Scientific™ SureTect™ Cronobacter Species PCR Assay.

Pricing & Availability

113

Thermo Scientific™ SureTect™ Escherichia coli O157:H7 and STEC Screening PCR Assay

Rapidly detect E. coli O157:H7, stx, eae, in food samples in as little as 10 hours with highly-specific, simple to use real-time PCR Thermo Scientific™ SureTect™ Escherichia coli O157:H7 and STEC Screening PCR Assay.

Pricing & Availability

114

Thermo Scientific™ T4 DNA Polymerase (5 U/μL)

Ensure accuracy in DNA synthesis with template-dependent T4 DNA Polymerase that catalyzes the 5'-3' synthesis from primed single-stranded DNA.

Pricing & Availability
Specifications

Polymerase	T4 DNA Polymerase
Concentration	5 U/μL

115

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Applied Biosystems™ TaqPath™ DuraPlex™ 1-Step RT-qPCR Master Mix

Applied Biosystems TaqPath DuraPlex 1-Step RT-qPCR Master Mix is a benchtop-stable, single-tube master mix optimized for rapid, sensitive, and reproducible detection of viral and bacterial pathogens even in the presence of PCR inhibitors.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	Medium
Content And Storage	Store at -15°C to -25°C. Store at room temperature (24°C) for 1 week after first use and 4°C for 1 month after first use.
Form	Liquid
Concentration	4X
For Use With (Application)	Microbial Detection,Gene Expression,DNA Quantification,Presence/Absence
PCR Method	1-step RT-qPCR
Multiplex Capability	Up to 5-plex
Detection Method	Primer-probe
Includes	dUTP
Thermostability	8 hr., Assembled Reaction
Polymerase	Applied Biosystems™ AmpliTaq™ DNA Polymerase with Hot Start
Format	Single-Tube
Passive Reference Dye	ROX (Pre-mixed)
Reaction Speed	Fast or Standard
Inhibitor Tolerance (Simple)	High
Target Specificity	RNA only, Both RNA and DNA
Hot Start	Antibody-based
Shelf Life	Two years from date of manufacture, with guaranteed minimum shelf life of ≥1 year upon receipt
Product Line	TaqPath

116

Applied Biosystems™ Detection Enhancer, for real-time PCR

Formulated to improve template amplification in real-time PCR (qPCR) workflows

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Product Type	Detection Enhancer
For Use With (Equipment)	7500 System
Sample Type	Viral Samples
Reaction Speed	Standard
For Use With (Application)	Real Time PCR (qPCR)
PCR Method	1-step RT-qPCR
Product Line	Applied Biosystems
Detection Method	Primer-probe

117

Thermo Scientific™ Taq Buffers (10X)

Thermo Scientific 10X Taq Buffers are ready-to-use buffers for PCR using Taq DNA Polymerases (both recombinant and native). Available in different compositions and also without detergents.

Pricing & Availability
Specifications

Physical Form	Liquid
Quantity	4 x 1.25 mL
Concentration	10X

118

Invitrogen™ Cells-to-CT™ 1-Step Power SYBR™ Green Kit

Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step Power SYBR™ Green Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

Pricing & Availability
Specifications

GC-Rich PCR Performance	High
Format	Tube
Product Type	1-Step qPCR kit
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type	Mammalian Cells
Reaction Speed	7 min.
Reagent Type	Cell Lysis
Starting Material	10-100,000 Cells
For Use With (Application)	Gene Expression
PCR Method	1-step RT-qPCR
Product Line	Cells-to-CT™, Power SYBR™ Green
Detection Method	SYBR

119

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Invitrogen™ ChargeSwitch™ PCR Clean-Up Kit

ChargeSwitch PCR Clean-Up Kit is the fastest and most effective method of removing primers, unincorporated dNTPs, thermostable polymerases and salts.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Target	DNA from Enzymatic Reactions (e.g. PCR)
Content And Storage	The ChargeSwitch™ PCR Clean-Up Kit is shipped at room temperature. Upon receipt, store all components at room temperature. All components are guaranteed stable for 6 months when stored properly. Components: • ChargeSwitch™ Magnetic Beads (25 mg/mL in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20) • ChargeSwitch™ Purification Buffer (N5) • ChargeSwitch™ Wash Buffer (W12) • ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
Format	Kit
Product Type	PCR Clean-Up Kit
Sample Type	PCR Products
Isolation Technology	ChargeSwitch™ Chemistry
Run Time	5 min
For Use With (Application)	Next-Generation Sequencing (NGS),Cloning,Standard PCR
High-throughput Compatibility	High-throughput Compatible
Product Line	ChargeSwitch

120

Thermo Scientific™ RevertAid H Minus First Strand cDNA Synthesis Kit

Thermo Scientific RevertAid H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Format	Kit
Reverse Transcriptase	RevertAid H Minus
Size (Final Product)	Up to 13 kb
Reaction Format	Separate components
Final Product Type	First-Strand cDNA
Reaction Speed	60 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Reduced
Optimal Reaction Temperature	42°C to 45°C
Technique	Reverse Transcription
Starting Material	RNA
For Use With (Application)	Real Time PCR (qPCR),RT-PCR

PCR Supplies

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Applied Biosystems™ ViralSEQ™ Lentivirus Physical Titer Kits Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Applied Biosystems™ AmpliTaq™ DNA Polymerase with Buffer I or Buffer II GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

Thermo Scientific™ T4 DNA Polymerase (5 U/μL) GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

Invitrogen™ Cells-to-CT™ 1-Step Power SYBR™ Green Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Applied Biosystems™ ViralSEQ™ Lentivirus Physical Titer Kits

Applied Biosystems™ AmpliTaq™ DNA Polymerase with Buffer I or Buffer II

Thermo Scientific™ T4 DNA Polymerase (5 U/μL)

Invitrogen™ Cells-to-CT™ 1-Step Power SYBR™ Green Kit